Which of the following is an example of manual restraint?

Manual restraint involves physically holding or controlling an animal to prevent it from moving freely, which is essential in various veterinary situations for the safety of both the animal and the handler. Using a leash exemplifies manual restraint as it allows the handler to control the animal's movement and position directly. This technique enables the handler to keep the animal close, preventing it from escaping or moving unpredictably, which is especially important during medical examinations or procedures.

While employing a muzzle, restraining with a game, and placing the animal in a crate are methods of control or prevention, they do not require direct physical interaction with the animal in the same way that using a leash does. A muzzle can prevent biting but does not control an animal's body movement; restraining with a game typically involves distractions rather than physical restraint; and placing an animal in a crate is more about confinement than direct manual control. Using a leash, therefore, stands out as the most direct form of manual restraint in the context provided.